Recombinant SARS-CoV-2 N Protein (T205I) (His Tag) | 763-PKSV030340

Recombinant SARS-CoV-2 N Protein (T205I) (His Tag) | PKSV030340 | Elabscience.

Synonym: N/A

Activity: Testing in progress

Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid (YP_009724397.2, with mutation T205I) (Met1-Ala419) was expressed with a polyhistidine tag at the N-terminus. The mutation were identified in the SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa.

Sequence: Met1-Ala419 (T205I)

Fusion tag: N-His

Accession: YP_009724397.2

Species: SARS-CoV-2

Expressed Host: E.coli

Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass: 46.6 kDa

AP Mol Mass: N/A

Formulation: Lyophilized from sterile 50 mM PB, 500 mM NaCl, pH 7.0.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.