Recombinant 2019-nCoV Spike Protein, Biotinylated (RBD-SD1, Avi-His Tag) | 763-PKSR030478

Recombinant 2019-nCoV Spike Protein, Biotinylated (RBD-SD1, Avi-His Tag) | PKSR030478 | Elabscience.

Synonym: 2019-nCov RBD Protein; 2019-nCoV S RBD Protein

Activity: 1.Immobilized Recombinant 2019-nCoV Spike Protein (RBD-SD1, His Tag) (Cat#PKSR030477) at 5μg/ml (100 μl/well) can bind Recombinant Human ACE-2 Protein (Fc Tag) (Cat#PKSR030492) .The ED50 of PKSR030492 is 76 ng/ml. 2.Immobilized Recombinant 2019-nCoV Spike Protein (RBD-SD1, His Tag) (Cat#PKSR030477) at 5μg/ml (100 μl/well) can bind SARS-COV-2 Spike S1 Monoclonal Antibody (Cat#E-AB-V1005) .The ED50 of E-AB-V1005 is 2.2 ng/ml.

Protein Construction: Biotinylated 2019-nCoV Spike Protein RBD-SD1 is produced by our Mammalian expression system and the target gene encoding Arg319-Ser591 is expressed with a Avi, 6His tag at the C-terminus.

Sequence: Arg319-Ser591

Fusion tag: C-Avi-6His

Accession: QHD43416.1

Species: SARS-CoV-2

Expressed Host: Human Cells

Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass: 33.2 kDa

AP Mol Mass: 38-45 kDa

Formulation: Supplied as a 0.2 μM filtered solution of PBS, pH 7.4.

Reconstitution: Not Applicable

Background: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS) . The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.