HCoV-229E Nucleocapsid protein, His Tag | NUN-V5147

HCoV-229E Nucleocapsid protein, His Tag | NUN-V5147 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins

Synonym

Nucleocapsid protein, NP, Protein N

Source

HCoV-229E Nucleocapsid, His Tag (NUN-V5147) is expressed from E.coli cells. It contains AA Met 1 - Asn 389 (Accession # P15130-1).
Predicted N-terminus: Met

Molecular Characterization

Online(Met 1 - Asn 389) P15130-1Development Suggestion
This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 44.4 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 40 mM PB, 500 mM NaCl, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.