Biotinylated SARS-CoV-2 Spike S1 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R), His, Avitag™ | S1N-C82E5

Biotinylated SARS-CoV-2 Spike S1 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R), His, Avitag™ | S1N-C82E5 | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1

Source

Biotinylated SARS-CoV-2 Spike S1, His,Avitag (S1N-C82E5) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). The mutations (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617.2).
Predicted N-terminus: Val 16

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 78.4 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.